MiR-144 as a novel biomarker in breast cancer diagnosis and treatment

Exosomes naturally carry the biomolecules in body; they perform this task efficiently without compromising immune system and by breaking through all biological barriers, so can be best choice for designing introducing a drug gene transfer system. Extraction of exosomes from cell culture medium was performed precipitation with an Exoquick kit solution. Nanoparticle specificity analysis using scanning electron microscopy dynamic light scattering. Trizol reagent (Invitrogen) used RNA extraction. Single-strand cDNA synthesis miRNA RT-PCR. Data were analyzed threshold cycle comparative method flow cytometry. containing miR-144 dramatically decrease expression level crucial TGF-β pathway genes, SMAD4 TGF-βR2, breast cancer cells. Botulinum toxin A inhibits growth inhibiting pathway. The simultaneous combination engineered bacterial botulinum has increased effects on signaling It causes arrest present study's findings showed that overexpression tumor cells results packaging derived these As result, exosomal platform nucleic acid to appears effective transducer cell. could as suitable adjunct therapeutic studies. Keywords: Breast cancer, A, exosome, miRNA, biomarker.